Water-deficit tolerance in citrus is mediated by the down regulation of PIP gene expression in the roots
Identifieur interne : 001917 ( Main/Exploration ); précédent : 001916; suivant : 001918Water-deficit tolerance in citrus is mediated by the down regulation of PIP gene expression in the roots
Auteurs : Juan Rodriguez-Gamir [Espagne] ; Gema Ancillo [Espagne] ; Frederic Aparicio [Espagne] ; Mireia Bordas [Espagne] ; Eduardo Primo-Millo [Espagne] ; M. Angeles Forner-Giner [Espagne]Source :
- Plant and soil [ 0032-079X ] ; 2011.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Agrume.
English descriptors
- KwdEn :
Abstract
Water deficit (WD) is a growing problem in agriculture. In citrus crops, genetically-determined root-stock characteristics are important factors influencing plant responses to WD. Aquaporins are involved in regulating the water supply to the plant by mediating water flow through the cell membranes. Recent studies support a direct role for aquaporins in plant water relations and demonstrate their involvement in WD tolerance. This study investigates the relationship between photosynthetic and water-balance parameters with aquaporin expression levels and hydraulic conductance of roots (Kr) in conditions of moderate WD in citrus rootstocks. The plant materials used were the rootstocks Poncirus trifoliata (L.) Raf. (PT), Cleopatra mandarin (Citrus reshni Hort ex Tan.) (CM) and 030115 (a hybrid of the two former rootstocks), all grafted with the citrus variety 'Valencia Late' (C. sinensis (L.) Osb). Plants were irrigated with two differents irrigation doses (normal irrigation and moderate WD) during 70 days and leaf water potential (ψs), net CO2 assimilation (ACO2), transpiration, stomatal conductance (gs) and substomatal CO2 concentration (Ci) were measured periodically under both irrigation conditions. Kr and PIP1 and PIP2 gene expression levels in fine roots of control plants and plants subjected to WD on day 43 of the experiment were determined. Under WD conditions, the hybrid 030115 drastically reduced aquaporin expression and Kr, accompanied by a loss of plant vigour but without reducing the net CO2 assimilation (ACO2). PT maintained the same aquaporin expression level and similar Kr under WD as under normal irrigation conditions, but suffered a sharp reduction in ACO2. CM, which has lower Kr and aquaporin expression than PT under both normal irrigation conditions and WD, responded better to water stress conditions than PT. Low aquaporin levels, or down-regulated aquaporin expression, accompanied by decreased plant vigour led to decreased plasma membrane permeability, thereby facilitating water retention in the cells under water stress conditions. This may induce water stress tolerance in citrus rootstocks.
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Water-deficit tolerance in citrus is mediated by the down regulation of PIP gene expression in the roots</title><author><name sortKey="Rodriguez Gamir, Juan" sort="Rodriguez Gamir, Juan" uniqKey="Rodriguez Gamir J" first="Juan" last="Rodriguez-Gamir">Juan Rodriguez-Gamir</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Carretera Moncada-Naquera, Km. 4.5, 46113, Moncada</s1><s2>Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Ancillo, Gema" sort="Ancillo, Gema" uniqKey="Ancillo G" first="Gema" last="Ancillo">Gema Ancillo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Carretera Moncada-Naquera, Km. 4.5, 46113, Moncada</s1><s2>Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Aparicio, Frederic" sort="Aparicio, Frederic" uniqKey="Aparicio F" first="Frederic" last="Aparicio">Frederic Aparicio</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia (CSIC-UPV), Edificio CPI, 8E, Avenida de los Naranjos, s/n</s1><s2>46022, Valencia</s2><s3>ESP</s3><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>46022, Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Bordas, Mireia" sort="Bordas, Mireia" uniqKey="Bordas M" first="Mireia" last="Bordas">Mireia Bordas</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Agromillora Iberia S.L., El Rebato s/n, 08739, Subirats</s1><s2>Barcelona</s2><s3>ESP</s3><sZ>4 aut.</sZ></inist:fA14><country>Espagne</country><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Primo Millo, Eduardo" sort="Primo Millo, Eduardo" uniqKey="Primo Millo E" first="Eduardo" last="Primo-Millo">Eduardo Primo-Millo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Carretera Moncada-Naquera, Km. 4.5, 46113, Moncada</s1><s2>Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Forner Giner, M Angeles" sort="Forner Giner, M Angeles" uniqKey="Forner Giner M" first="M. 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Angeles Forner-Giner</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Carretera Moncada-Naquera, Km. 4.5, 46113, Moncada</s1><s2>Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">11-0440571</idno><date when="2011">2011</date><idno type="stanalyst">PASCAL 11-0440571 INIST</idno><idno type="RBID">Pascal:11-0440571</idno><idno type="wicri:Area/PascalFrancis/Corpus">000145</idno><idno type="wicri:Area/PascalFrancis/Curation">000855</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000134</idno><idno type="wicri:doubleKey">0032-079X:2011:Rodriguez Gamir J:water:deficit:tolerance</idno><idno type="wicri:Area/Main/Merge">001943</idno><idno type="wicri:Area/Main/Curation">001917</idno><idno type="wicri:Area/Main/Exploration">001917</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Water-deficit tolerance in citrus is mediated by the down regulation of PIP gene expression in the roots</title><author><name sortKey="Rodriguez Gamir, Juan" sort="Rodriguez Gamir, Juan" uniqKey="Rodriguez Gamir J" first="Juan" last="Rodriguez-Gamir">Juan Rodriguez-Gamir</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Carretera Moncada-Naquera, Km. 4.5, 46113, Moncada</s1><s2>Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Ancillo, Gema" sort="Ancillo, Gema" uniqKey="Ancillo G" first="Gema" last="Ancillo">Gema Ancillo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Carretera Moncada-Naquera, Km. 4.5, 46113, Moncada</s1><s2>Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Aparicio, Frederic" sort="Aparicio, Frederic" uniqKey="Aparicio F" first="Frederic" last="Aparicio">Frederic Aparicio</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia (CSIC-UPV), Edificio CPI, 8E, Avenida de los Naranjos, s/n</s1><s2>46022, Valencia</s2><s3>ESP</s3><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>46022, Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Bordas, Mireia" sort="Bordas, Mireia" uniqKey="Bordas M" first="Mireia" last="Bordas">Mireia Bordas</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Agromillora Iberia S.L., El Rebato s/n, 08739, Subirats</s1><s2>Barcelona</s2><s3>ESP</s3><sZ>4 aut.</sZ></inist:fA14><country>Espagne</country><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Primo Millo, Eduardo" sort="Primo Millo, Eduardo" uniqKey="Primo Millo E" first="Eduardo" last="Primo-Millo">Eduardo Primo-Millo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Carretera Moncada-Naquera, Km. 4.5, 46113, Moncada</s1><s2>Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Forner Giner, M Angeles" sort="Forner Giner, M Angeles" uniqKey="Forner Giner M" first="M. Angeles" last="Forner-Giner">M. Angeles Forner-Giner</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Carretera Moncada-Naquera, Km. 4.5, 46113, Moncada</s1><s2>Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Plant and soil</title><title level="j" type="abbreviated">Plant soil</title><idno type="ISSN">0032-079X</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant and soil</title><title level="j" type="abbreviated">Plant soil</title><idno type="ISSN">0032-079X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aquaporin</term><term>Citrus</term><term>Citrus fruit</term><term>Gene expression</term><term>Hydraulic conductivity</term><term>Photosynthesis</term><term>Regulation(control)</term><term>Root</term><term>Rootstock</term><term>Soil plant relation</term><term>Tolerance</term><term>Transpiration</term><term>Water deficit</term><term>Water stress</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Déficit hydrique</term><term>Tolérance</term><term>Expression génique</term><term>Régulation</term><term>Porte greffe</term><term>Aquaporine</term><term>Racine</term><term>Conductivité hydraulique</term><term>Photosynthèse</term><term>Citrus</term><term>Agrume</term><term>Transpiration</term><term>Stress hydrique</term><term>Relation sol plante</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Agrume</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Water deficit (WD) is a growing problem in agriculture. In citrus crops, genetically-determined root-stock characteristics are important factors influencing plant responses to WD. Aquaporins are involved in regulating the water supply to the plant by mediating water flow through the cell membranes. Recent studies support a direct role for aquaporins in plant water relations and demonstrate their involvement in WD tolerance. This study investigates the relationship between photosynthetic and water-balance parameters with aquaporin expression levels and hydraulic conductance of roots (Kr) in conditions of moderate WD in citrus rootstocks. The plant materials used were the rootstocks Poncirus trifoliata (L.) Raf. (PT), Cleopatra mandarin (Citrus reshni Hort ex Tan.) (CM) and 030115 (a hybrid of the two former rootstocks), all grafted with the citrus variety 'Valencia Late' (C. sinensis (L.) Osb). Plants were irrigated with two differents irrigation doses (normal irrigation and moderate WD) during 70 days and leaf water potential (ψs), net CO<sub>2</sub> assimilation (A<sub>CO2</sub>), transpiration, stomatal conductance (gs) and substomatal CO<sub>2</sub> concentration (Ci) were measured periodically under both irrigation conditions. Kr and PIP1 and PIP2 gene expression levels in fine roots of control plants and plants subjected to WD on day 43 of the experiment were determined. Under WD conditions, the hybrid 030115 drastically reduced aquaporin expression and Kr, accompanied by a loss of plant vigour but without reducing the net CO<sub>2</sub> assimilation (A<sub>CO2</sub>). PT maintained the same aquaporin expression level and similar Kr under WD as under normal irrigation conditions, but suffered a sharp reduction in A<sub>CO2</sub>. CM, which has lower Kr and aquaporin expression than PT under both normal irrigation conditions and WD, responded better to water stress conditions than PT. Low aquaporin levels, or down-regulated aquaporin expression, accompanied by decreased plant vigour led to decreased plasma membrane permeability, thereby facilitating water retention in the cells under water stress conditions. This may induce water stress tolerance in citrus rootstocks.</div></front></TEI><affiliations><list><country><li>Espagne</li></country><region><li>Catalogne</li></region><settlement><li>Barcelone</li></settlement></list><tree><country name="Espagne"><noRegion><name sortKey="Rodriguez Gamir, Juan" sort="Rodriguez Gamir, Juan" uniqKey="Rodriguez Gamir J" first="Juan" last="Rodriguez-Gamir">Juan Rodriguez-Gamir</name></noRegion><name sortKey="Ancillo, Gema" sort="Ancillo, Gema" uniqKey="Ancillo G" first="Gema" last="Ancillo">Gema Ancillo</name><name sortKey="Aparicio, Frederic" sort="Aparicio, Frederic" uniqKey="Aparicio F" first="Frederic" last="Aparicio">Frederic Aparicio</name><name sortKey="Bordas, Mireia" sort="Bordas, Mireia" uniqKey="Bordas M" first="Mireia" last="Bordas">Mireia Bordas</name><name sortKey="Forner Giner, M Angeles" sort="Forner Giner, M Angeles" uniqKey="Forner Giner M" first="M. Angeles" last="Forner-Giner">M. Angeles Forner-Giner</name><name sortKey="Primo Millo, Eduardo" sort="Primo Millo, Eduardo" uniqKey="Primo Millo E" first="Eduardo" last="Primo-Millo">Eduardo Primo-Millo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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